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  Indian J Med Microbiol
 

Figure 2: BPA was conjugated to BSA for specificity of the BPA antibody to identify BPA. (a) Amido black staining. Lane 1, 2 μg BSA. Lane 2, 0.2 μg BSA conjugated to BPA. (b) Immunoblot using goat anti-BPA antibody raised against a BPA-keyhole limpet hemocyanin (KLH) antigen. Lane 1, 2 μg BSA. Lane 2, 0.2 μg BSA conjugated to BPA (arrow indicates to the correct molecular weight of monomeric BPA-BSA conjugate). BSA polymers were formed during BPA conjugation to BSA. BPA: Bisphenol A, BSA: Bovine serum albumin

Figure 2: BPA was conjugated to BSA for specificity of the BPA antibody to identify BPA. (a) Amido black staining. Lane 1, 2 μg BSA. Lane 2, 0.2 μg BSA conjugated to BPA. (b) Immunoblot using goat anti-BPA antibody raised against a BPA-keyhole limpet hemocyanin (KLH) antigen. Lane 1, 2 μg BSA. Lane 2, 0.2 μg BSA conjugated to BPA (arrow indicates to the correct molecular weight of monomeric BPA-BSA conjugate). BSA polymers were formed during BPA conjugation to BSA. BPA: Bisphenol A, BSA: Bovine serum albumin