| ORIGINAL ARTICLE |
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| Year : 2020 | Volume
: 5
| Issue : 3 | Page : 78-82 |
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Quantitative estimation of urinary ortho-cresol by high pressure liquid chromatography for biomonitoring of toluene exposed population
Anupa Yadav, Amit Chakrabarti, Geoffrey Nengzapum
Department of Industrial Hygiene, Regional Occupational Health Centre (Eastern), Kolkata, West Bengal, India
Correspondence Address:
Ms. Anupa Yadav
Department of Industrial Hygiene, Regional Occupational Health Centre (Eastern), Block DP, Sector V, Salt Lake, Kolkata - 700 091, West Bengal
India
 Source of Support: None, Conflict of Interest: None  | Check |
DOI: 10.4103/ed.ed_23_20
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Aim: Quantification of urinary ortho-cresol (OC) by high pressure liquid chromatography coupled with photodiode array detector.
Materials and Methods: Includes acid hydrolysis of urine, liquid–liquid extraction, and chromatography quantification of extracted OC in urine.
Results: Limit of detection, limit of quantification, and coefficient of linearity (R2) were 0.18 μg/ml, 0.62 μg/ml, and 0.9998, respectively. Recovery % of method ranged from 92%, 97%, and 100%. For intraday and interday precision coefficient of variation was 0.41%, 0.64%, and 0.89%, 0.86% for urine samples spiked with OC standards final concentration of 0.25 μg/ml and 0.7 μg/ml, respectively. Results (mean ± standard deviation) of exposed and unexposed real urine samples analyzed for OC with this method were 0.92 ± 0.76 and 0.40 ± 0.20 μg/ml, respectively. Statistical analysis of results showed significant (P ≤ 0.001) difference between urinary OC among exposed and unexposed subjects.
Conclusion: The present work describes precise, easy, and less time consuming method for estimation of OC in urine of population exposed to toluene. It can be used as a promising tool for biomonitoring of population exposed to toluene.
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